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Immunofixation (IFE), Serum and Protein Electrophoresis, Serum
CPT:
82784(x3) (IgG, IgA, IgM); 84155; 84165 (protein electrophoretic fractionation and quantitation); 86334 (immunofixation electrophoresis)
Synonyms
- Globulins, Serum
- IEP, Serum
- IFE, Serum and Protein Electrophoresis, Serum
- Immunoelectrophoresis, Serum
- Protein IEP
Test Includes
Quantitation of immunoglobulins IgA, IgG, and IgM; IFE interpretation of heavy- and light-chain patterns; serum protein electrophoresis
Special Instructions
State the patient's age and clinical diagnosis on the test request form.
Expected Turnaround Time
3 - 5 days
Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Related Information
Related Documents
For more information, please view the literature below.
Specimen Requirements
Specimen
Serum
Volume
5 mL
Minimum Volume
2 mL (Note: This volume does not allow for repeat testing.)
Container
Red-top tube or gel-barrier tube
Collection
Separate serum from cells.
Storage Instructions
Refrigerate.
Stability Requirements
Temperature | Period |
|---|---|
Room temperature | 3 days |
Refrigerated | 14 days |
Frozen | 14 days |
Freeze/thaw cycles | Stable x3 |
Patient Preparation
Overnight fasting is preferred, but not required.
Causes for Rejection
Plasma sample
Test Details
Use
Detect and identify monoclonal immunoglobulin gammopathies
Methodology
Electrophoresis followed by immunodiffusion against monospecific antisera to immunoglobulin and individual heavy and light chains (IFE); protein electrophoresis on agarose media; turbidimetric quantitation of IgA, IgG, and IgM
Reference Interval
Normal pattern
Additional Information
Diagnose myeloma, macroglobulinemia of Waldenström. Immunofixation is used to evaluate monoclonal gammopathy (M protein) found on serum protein electrophoresis; evaluate amyloidosis. Has application to the evaluation of lymphoproliferative disease (malignant lymphoma and others) and collagen disease in general. Of use in the diagnosis and characterization of immune deficient and dysgammaglobulinemic states.
LOINC® Map
| Order Code | Order Code Name | Order Loinc | Result Code | Result Code Name | UofM | Result LOINC |
|---|---|---|---|---|---|---|
| 001495 | IFE and PE, Serum | 001776 | Immunoglobulin G, Qn, Serum | mg/dL | 2465-3 | |
| 001495 | IFE and PE, Serum | 001784 | Immunoglobulin A, Qn, Serum | mg/dL | 2458-8 | |
| 001495 | IFE and PE, Serum | 001792 | Immunoglobulin M, Qn, Serum | mg/dL | 2472-9 | |
| 001495 | IFE and PE, Serum | 001073 | Protein, Total | g/dL | 2885-2 | |
| 001495 | IFE and PE, Serum | 149520 | Albumin | g/dL | 2862-1 | |
| 001495 | IFE and PE, Serum | 149521 | Alpha-1-Globulin | g/dL | 2865-4 | |
| 001495 | IFE and PE, Serum | 149522 | Alpha-2-Globulin | g/dL | 2868-8 | |
| 001495 | IFE and PE, Serum | 149523 | Beta Globulin | g/dL | 2871-2 | |
| 001495 | IFE and PE, Serum | 149524 | Gamma Globulin | g/dL | 2874-6 | |
| 001495 | IFE and PE, Serum | 149525 | M-Spike | g/dL | 33358-3 | |
| 001495 | IFE and PE, Serum | 149530 | Globulin, Total | g/dL | 2336-6 | |
| 001495 | IFE and PE, Serum | 149531 | A/G Ratio | 1759-0 | ||
| 001495 | IFE and PE, Serum | 149519 | Immunofixation Result, Serum | 13169-8 | ||
| 001495 | IFE and PE, Serum | 149534 | Please note: | 77202-0 | ||
| 001495 | IFE and PE, Serum | 001498 | 11502-2 |
| Reflex Table for Immunofixation Result, Serum | ||||||
|---|---|---|---|---|---|---|
| Order Code | Order Name | Result Code | Result Name | UofM | Result LOINC | |
| Reflex 1 | 149532 | Immunofixation Interp.,Sr | 149532 | Immunofixation Interp.,Sr | 13169-8 | |
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The LOINC® codes are copyright © 1994-2021, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee. Permission is granted in perpetuity, without payment of license fees or royalties, to use, copy, or distribute the LOINC® codes for any commercial or non-commercial purpose, subject to the terms under the license agreement found at https://loinc.org/license/. Additional information regarding LOINC® codes can be found at LOINC.org, including the LOINC Manual, which can be downloaded at LOINC.org/downloads/files/LOINCManual.pdf