Skip to main content

Antibody Screen

CPT 86850
Synonyms
  • Indirect Antiglobulin Test
  • Indirect Coombs'

Test Details

Methodology

Antiglobulin test

Result Turnaround Time

1 - 2 days

Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Related Documents

Test Includes

Red cell antibody screening. Note: Identification of antibody(ies) and titer of clinically significant antibody(ies) will be automatically performed at an additional charge if the antibody screen is positive.

Use

Detect atypical IgG antibodies prior to transfusion or during pregnancy. The technique is designed specifically to detect IgG antibodies, though on occasion, some IgM antibodies may also be detected. Antibodies detected by the antibody screen will be subsequently identified, and a titer performed if the antibody identified is considered to be clinically significant during pregnancy.

Limitations

No antibody screening test will detect all antibodies. This method may not detect IgM antibodies or antibodies to low frequency antigens. Extremely low-titered antibodies may be detected but may be too weak to identify.

Contraindications

Specimens known to test positive for red cell antibody screening should not be submitted for antibody screen testing. In order to facilitate proper routing and testing, Antibody Identification (006213) should be ordered. This test should not be ordered for the husband or partner of a prenatal patient since antibodies detected in the serum or plasma of these individuals are irrelevant during pregnancy.

References

Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.

Specimen Requirements

Specimen

Whole blood (Note: This should not be a shared specimen. If antibody identification is necessary, the entire volume of this lavender-top tube will be required.)

Volume

4 mL

Minimum Volume

1 mL (Note: This minimum amount will not allow for antibody identification if the antibody screen is positive.)

Container

Lavender-top (EDTA) tube

Reference Range

Negative

Storage Instructions

Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze red cells.

Causes for Rejection

Gross hemolysis; bacterial or other contamination

LOINC® Map

Order Code Order Code Name Order Loinc Result Code Result Code Name UofM Result LOINC
006015 Antibody Screen 890-4 006015 Antibody Screen 890-4
Order Code006015
Order Code NameAntibody Screen
Order Loinc890-4
Result Code006015
Result Code NameAntibody Screen
UofM
Result LOINC890-4
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 114033 Antibody Screen 890-4
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code114033
Result NameAntibody Screen
UofM
Result LOINC890-4
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019786 Antibody Id. #1 888-8
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019786
Result NameAntibody Id. #1
UofM
Result LOINC888-8
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019786 Antibody Id. #1 888-8
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019786
Result NameAntibody Id. #1
UofM
Result LOINC888-8
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019786 Antibody Id. #1 888-8
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019786
Result NameAntibody Id. #1
UofM
Result LOINC888-8
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019786 Antibody Id. #1 888-8
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019786
Result NameAntibody Id. #1
UofM
Result LOINC888-8
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019786 Antibody Id. #1 888-8
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019786
Result NameAntibody Id. #1
UofM
Result LOINC888-8
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019802 Coombs Titer #1 50970-3
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019802
Result NameCoombs Titer #1
UofM
Result LOINC50970-3
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019810 Antibody Id. #2 888-8
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019810
Result NameAntibody Id. #2
UofM
Result LOINC888-8
Reflex Table for Antibody Screen
Order Code Order Name Result Code Result Name UofM Result LOINC
Reflex 1 114041 Ab Scr+Antibody ID 019836 Coombs Titer #2 50970-3
Reflex 1
Order Code114041
Order NameAb Scr+Antibody ID
Result Code019836
Result NameCoombs Titer #2
UofM
Result LOINC50970-3